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Growth performance and intestinal Clostridium perfringens quantification. (A) Average body weight (BW, kg/bird) measured at 21 d and 35 d of age across different treatment groups. (B) Average daily gain (ADG, g/bird/d) from 21 d to 35 d of age. (C) Amplification plot derived from serial dilutions of C. perfringens ML2 <t>genomic</t> <t>DNA.</t> (D) Melting curve analysis confirming the specific amplification of the target gene segment. (E) Standard curve analysis of a standard plasmid containing a cpa gene. The concentrations ranged from 2.60 × 10 8 to 2.60 × 10 1 copies/μL; y = -3.244x + 37.28; Correlation coefficient (R2) = 0.9893. (F) Copies of ML2 genomic DNA in the intestinal contents.
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Growth performance and intestinal Clostridium perfringens quantification. (A) Average body weight (BW, kg/bird) measured at 21 d and 35 d of age across different treatment groups. (B) Average daily gain (ADG, g/bird/d) from 21 d to 35 d of age. (C) Amplification plot derived from serial dilutions of C. perfringens ML2 <t>genomic</t> <t>DNA.</t> (D) Melting curve analysis confirming the specific amplification of the target gene segment. (E) Standard curve analysis of a standard plasmid containing a cpa gene. The concentrations ranged from 2.60 × 10 8 to 2.60 × 10 1 copies/μL; y = -3.244x + 37.28; Correlation coefficient (R2) = 0.9893. (F) Copies of ML2 genomic DNA in the intestinal contents.
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  Buy from Supplier
bacterial genomic dna extraction kit  (tiangen biotech co)
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Growth performance and intestinal Clostridium perfringens quantification. (A) Average body weight (BW, kg/bird) measured at 21 d and 35 d of age across different treatment groups. (B) Average daily gain (ADG, g/bird/d) from 21 d to 35 d of age. (C) Amplification plot derived from serial dilutions of C. perfringens ML2 <t>genomic</t> <t>DNA.</t> (D) Melting curve analysis confirming the specific amplification of the target gene segment. (E) Standard curve analysis of a standard plasmid containing a cpa gene. The concentrations ranged from 2.60 × 10 8 to 2.60 × 10 1 copies/μL; y = -3.244x + 37.28; Correlation coefficient (R2) = 0.9893. (F) Copies of ML2 genomic DNA in the intestinal contents.
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https://www.bioz.com/result/bacterial genomic dna extraction kit/product/tiangen biotech co
Average 96 stars, based on 1 article reviews
bacterial genomic dna extraction kit - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier
bacterial genome extraction kit  (tiangen biotech co)
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Growth performance and intestinal Clostridium perfringens quantification. (A) Average body weight (BW, kg/bird) measured at 21 d and 35 d of age across different treatment groups. (B) Average daily gain (ADG, g/bird/d) from 21 d to 35 d of age. (C) Amplification plot derived from serial dilutions of C. perfringens ML2 <t>genomic</t> <t>DNA.</t> (D) Melting curve analysis confirming the specific amplification of the target gene segment. (E) Standard curve analysis of a standard plasmid containing a cpa gene. The concentrations ranged from 2.60 × 10 8 to 2.60 × 10 1 copies/μL; y = -3.244x + 37.28; Correlation coefficient (R2) = 0.9893. (F) Copies of ML2 genomic DNA in the intestinal contents.
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Growth performance and intestinal Clostridium perfringens quantification. (A) Average body weight (BW, kg/bird) measured at 21 d and 35 d of age across different treatment groups. (B) Average daily gain (ADG, g/bird/d) from 21 d to 35 d of age. (C) Amplification plot derived from serial dilutions of C. perfringens ML2 genomic DNA. (D) Melting curve analysis confirming the specific amplification of the target gene segment. (E) Standard curve analysis of a standard plasmid containing a cpa gene. The concentrations ranged from 2.60 × 10 8 to 2.60 × 10 1 copies/μL; y = -3.244x + 37.28; Correlation coefficient (R2) = 0.9893. (F) Copies of ML2 genomic DNA in the intestinal contents.

Journal: Poultry Science

Article Title: Optimization of culture conditions for enhanced antibacterial metabolite production by Bacillus velezensis TL and evaluation of its efficacy against necrotic enteritis in broilers

doi: 10.1016/j.psj.2026.106439

Figure Lengend Snippet: Growth performance and intestinal Clostridium perfringens quantification. (A) Average body weight (BW, kg/bird) measured at 21 d and 35 d of age across different treatment groups. (B) Average daily gain (ADG, g/bird/d) from 21 d to 35 d of age. (C) Amplification plot derived from serial dilutions of C. perfringens ML2 genomic DNA. (D) Melting curve analysis confirming the specific amplification of the target gene segment. (E) Standard curve analysis of a standard plasmid containing a cpa gene. The concentrations ranged from 2.60 × 10 8 to 2.60 × 10 1 copies/μL; y = -3.244x + 37.28; Correlation coefficient (R2) = 0.9893. (F) Copies of ML2 genomic DNA in the intestinal contents.

Article Snippet: Approximately 0.20 g of each sample was transferred into a 1.5 mL microcentrifuge tube, and bacterial genomic DNA was extracted via the TIANamp Stool DNA Kit (Cat. No. DP328-02; Tiangen Biotech, Beijing, China) according to the manufacturer’s instructions.

Techniques: Amplification, Derivative Assay, Plasmid Preparation